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That's 10 times more than its predecessor could, but it may seem small by later this year, when Affymetrix plans to release a 500K SNP chip.Real-time quantitative PCR (q PCR) is a powerful tool for quantifying specific DNA target sequences.Quantitative PCR is a rapid and highly sensitive technique for accurate quantification of microarray results; however, careful consideration of experimental design, quality of primer/probe design, internal standards, and normalization procedures are pivotal, particularly when the work involves postmortem tissue.In the late 1990s, you could measure the market in DNA microarrays in the tens of millions of dollars.The most traditional method for characterizing m RNA transcripts is northern blot analysis.We compared the accuracy of microarray measurements obtained with oligonucleotide arrays (Gene Chip, Affymetrix) with a laboratory-developed c DNA array by assaying test RNA samples from an experiment using a paradigm known to regulate many genes measured on both arrays.

Frost & Sullivan, San Antonio, Texas, which analyzes emerging high-tech and industrial markets, projects a world microarray market of over 0 million this year, and predicts that it will be closing in on a billion dollars annually by 2010.

We selected 47 genes represented on both arrays, including both known regulated and unregulated transcripts, and established reference relative expression measurements for these genes in the test RNA samples using quantitative reverse transcriptase real-time PCR (QRTPCR) assays.

The validity of the reproducible (average coefficient of variation = 11.8%) QRTPCR measurements were established through application of a new mathematical model.

Micro RNAs (mi RNAs) represent a growing class of small non-coding RNAs that are important regulators of gene expression in both plants and animals.

Studies have shown that mi RNAs play a critical role in human cancer and they can influence the level of cell proliferation and apoptosis by modulating gene expression.




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